J. Schmalfuss et al., Inhibition of acyl-CoA elongation by chloroacetamide herbicides in microsomes from leek seedlings, PEST BIOCH, 67(1), 2000, pp. 25-35
The inhibition of very-long-chain fatty acid (VLCFA) synthesis by chloroace
tamide herbicides was studied in vivo as well as in vitro. Incorporation of
[2-C-14]malonate into C-20 to C-24 VLCFAs Of etiolated leek seedlings was
strongly inhibited by the chloroacetamide metazachlor (I-50 approximate to
10-100 nM). The I-50 values of inhibition of incorporation into each VLCFA
decreased with their chain length [I-50(20:0) approximate to 1 mu M, I-50(2
2:0) approximate to 100 nM I-50(24:0) approximate to 10 nM]. With a microso
mal preparation from leek seedlings an in vitro elongase assay to quantify
chloroacetamide activity was developed using [2-C-14]malonyl-CoA and acyl-C
oA (16:0-CoA to 22:0-CoA) as substrates. Under our assay conditions the I-5
0 values of inhibition by metazachlor of each step of acyl-CoA elongation w
ere determined to be 0.5 mu M (for elongation of 16:0-CoA), 1.7 mu M (18:0-
CoA), 0.7 mu M (20:0-CoA), and 0.5 mu M (22:0-CoA). Furthermore, it could b
e shown that inhibition increased with decreasing concentrations of acyl-Co
A primer substrate. Using the chiral chloroacetamide metolachlor acyl-CoA e
longation was only inhibited with the herbicidally active S-enantiomer whil
e the R-enantiomer had no influence. As demonstrated by 20:0-CoA elongation
the I50 value decreased about 10-fold after preincubation of the enzyme pr
eparation at higher temperatures. Furthermore, enzyme activity could not be
recovered by dilution of the enzyme-inhibitor complex. These findings stro
ngly support the hypothesis that a covalent binding of metazachlor to its t
arget may occur. From this study the mode of action of herbicidal chloroace
tamides is disclosed as a strong inhibition of VLCFA synthesis resulting fr
om (a) an inhibition of each elongation step in series, (b) the increase of
that inhibition with the decrease of acyl-CoA primer substrate concentrati
on, and ic) the tight binding of the inhibitor to the target enzyme. (C) 20
00 Academic Press.