Plant regeneration of mangosteen via nodular callus formation

Nodular callus was induced at a high frequency on young purple red, 5-15 mm long laminae taken from in vitro grown plants of mangosteen. The optimal m edium was composed of Murashige and Skoog (MS) nutrients supplemented with 2.22 mu M benzyladenine (BA), 2.25 mu M thidiazuron (TDZ), 500 mg l(-1) pol yvinylpyrrolidone (PVP 360 000) and 3% sucrose. A multiplication rate of tw o-three was obtained by subculture of the nodular callus at 3-4-week interv als. Plantlet regeneration from the nodules was achieved by transfer to woo dy plant medium (WPM) with 500 mg l(-1) PVP, 0.4 mu M BA and 3% sucrose and overlaying with half strength liquid MS containing 0.32 mu M naphthaleneac etic acid (NAA), 0.13 mu M BA and 3% sucrose. Elongated shoots were rooted to 100% when wounded at the base of shoot, dipped in 4.4 mM indolebutyric a cid (IBA) solution in the dark for 15 min and cultured on WPM supplemented with 1.11 mu M BA, 0.25% activated charcoal, 34.5 mu M phloroglucinol (PG) and 3% sucrose.