Intracellular parasitism by the human granulocytic ehrlichiosis bacterium through the P-selectin ligand, PSGL-1

Human granulocytic ehrlichiosis (HGE) is a febrile tick-borne illness cause d by a recently discovered intracellular bacterium remarkable for its tropi sm for professionally phagocytic neutrophils. Monoclonal antibodies against the P-selectin binding domain of the leukocyte P-selectin glycoprotein lig and, PSGL-1, prevented HGE cell binding and infection, as did enzymatic dig estion of PSGL-1. Furthermore, simultaneous neoexpression in nonsusceptible cells of complementary DNAs for both PSGL-1 and its modifying alpha-(1,3) fucosyltransferase, Fuc-TVII, allowed binding and infection by HGE. Thus, t he HGE bacterium specifically bound to fucosylated Leukocyte PSGL-1. Select in mimicry is likely central to the organism's unique ability to target and infect neutrophils.