Determination of antibacterial reagents by liquid chromatography-electrospray-mass spectrometry

This paper describes a newly developed liquid chromatography-electrospray-m ass spectrometry (HPLC-ES-MS) method for the determination of 13 antibacter ial reagents (olaquindox, trimethoprim, clopidol, ormethoprim, morantel, ca rbadox, thiamphenicol, pyrimethamine, furazolidone, oxolinic acid, difurazo n, nalidixic acid, piromidic acid). The optimization for the detection of t hese compounds by HPLC-ES-MS was investigated. A C-18 column with gradient elution was utilized for the separation of thirteen antibacterial chemicals . Collision induced dissociation (CID) was used to induce fragmentation of analyte molecules and enhance the specificity of the method. Selective ion monitoring (SIM) was employed for quantitative determination. The detection limit of this method proved to be much better than previously reported one s. Satisfactory linearity, 0.5-10 ppm, of each compound was obtained. A. so lvent extraction method to extract analyte compound from pork was developed . The application of this newly developed method was demonstrated by analyz ing antibacterial reagent added pork samples. (C) 2000 Elsevier Science B.V . All rights reserved.