Cellulase enzymes have been widely used in denim processing to produce fash
ionably aged looks. Conventional whole acid stable cellulases are being use
d successfully to achieve a worn look with short process times. The final l
ook achieved by these types of enzymes is usually characterized as flat and
low contrast, due to a high level of backstaining. Literature reports sugg
est that backstaining is caused by many of the proteins present in the conv
entional whole acid stable cellulases. In this study, laboratory scale wash
performance tests were conducted using a unique protease under different p
rocessing conditions. The protease added during rinsing or at the end of th
e cellulase washing step resulted in significant reduction of backstaining
and improved contrast. The protease treatment at low temperatures also remo
ved all but trace amounts of residual cellulase bound to the denim.