Cloning and expression of the platelet-specific collagen receptor glycoprotein VI

Platelet glycoprotein VI (GP VI) was purified from platelet membranes and i ts internal amino acid sequences were determined. The cloned cDNA of GP VI indicates an open reading frame coding for 20 amino acid signal sequences a nd a mature protein of 319 amino acids. Its extracellular region has two Ig -like domains and a mucin-like, Ser/Thr-rich region, suggesting that GP VI is a member of the paired Ig-like receptor family. GP VI-transfected cells contained convulxin-(reactive) and antibody against recombinant GP VI-react ive protein bands that migrated at the same position as platelet GP VI in S DS/PAGE-electroblotting. These data indicate that the protein deduced from the cloned cDNA corresponds to platelet CP VI. (C) 2000 Elsevier Science Lt d. All rights reserved.