THE EVIDENCE OF CLONAL EVOLUTION WITH MONOSOMY-7 IN APLASTIC-ANEMIA FOLLOWING GRANULOCYTE-COLONY-STIMULATING-FACTOR USING THE POLYMERASE-CHAIN-REACTION

We present here the case of a Japanese female patient with aplastic an emia who developed monosomy 7 and clonal evolution following a treatme nt with recombinant human granulocyte colony-stimulating factor (rhG-C SF). At the onset of aplastic anemia, cytogenetic analysis was 46, XX and X-inactivation/methylation analysis revealed a polyclonal pattern, After 4 months of administration of rhG-CSF, she had 45, XX, -7 and a clonal pattern, although there were no morphological evidence of a my elodysplastic syndrome or leukemia, The ratio of monosomy 7 to normal analyzed by fluorescence in situ hybridization decreased after discont inuation of rhG-CSF and there were still no dysplastic changes and/or increased numbers of blasts, These results indicate that the acquisiti on of monosomy 7 following rhG-CSF treatment dose not always cause clo nal evolution to induce hematological malignancies.