Virus infection in patients with histiocytic necrotizing lymphadenitis in Taiwan - Detection of Epstein-Barr virus, type I human T-cell lymphotropic virus, and parvovirus B19

The relationship of Epstein-Barr virus (EBV), type I human T-cell lymphotro pic virus (HTLV-I), and parovirus B19 to histiocytic necrotizing lymphadeni tis was studied prospectively in 10 Taiwanese patients using materials obta ined by fine-needle aspiration and lymh node biopsy. The presence of EBV wa s detected by in situ hybridization foe EBV-encoced RNA expression. Immunoc ytochemistry was used to detect virus-encoded protein for EBV and parovirus B19. DNA in situ hybridization and polymerase chain reaction were performe d to determine the existence of HTLV-I provirus. Expressions of EBV-encoded RNA and Fas ligand were detected in all cases. Expression of EBV-encoded p rotein was identified in only 1 case. Neither HTLV-I nor parovirus B19 was detected in any case.