Salivary gland carcinomas displaying exclusively myoepithelial differentiat
ion (myoepithelial carcinoma) are considered rare. Their histopathologic fe
atures, immunohistochemical profile, and clinical behavior are not well cha
racterized. The authors reviewed the clinicopathologic features of 25 saliv
ary gland tumors fulfilling two fundamental histologic criteria: unequivoca
lly malignant and exclusively myoepithelial. For most of these, the origina
l diagnosis was malignant mixed tumor. Thirteen men and 12 women aged 24 to
77 years (mean age, 55 yrs) participated in the study, and most presented
with a painless mass. The parotid gland was the most common site (n = 15).
Tumors ranged from 2.1 to 5.5 cm, arising either in association with a beni
gn mixed tumor (n = 15) or de novo (n = 10). Histologically, all the tumors
displayed infiltrative growth and most had a characteristic multinodular a
rchitecture with a cellular periphery and central necrotic/myxoid zones. Ep
ithelioid, hyaline, spindle, clear, or mixed cell types were noted with acc
ompanying myxoid and/or hyalinized extracellular matrix. Ten tumors were hi
gh grade cytologically and 15 were low grade. The mitotic rate ranged from
three to 51 mitoses per 10 high-power fields. Necrosis was present in 15 tu
mors and perineural and vascular invasion were identified in 11 and four ne
oplasms respectively. Immunoreactivities included CAM5.2 (89%), AE1:AE3 (10
0%), 34 beta E12 (92%), cytokeratin 7 (21%), cytokeratin 14 (53%), vimentin
(100%), S-100 protein(100%), smooth muscle actin (50%), calponin (75%), mu
scle-specific actin (31%), glial fibrillary acidic protein (31%), carcinoem
bryonic antigen (0%), and epithelial membrane antigen (21%). Ultrastructura
l examination of three tumors showed myoepithelial features. Ten patients d
eveloped recurrences, mostly multiple. Follow up of 17 patients showed that
eight patients (47%) developed metastases (six high grade, two low grade)
and five patients (29%) died of disease (four high grade, one low grade) af
ter a mean of 32 months. Two patients were alive with disease (19 and 49 mo
s). Ten patients (59%) were without any evidence of disease after a mean of
42.2 months. Myoepithelial carcinomas exhibit a wide spectrum of cytomorph
ologic features and diverse clinical outcomes. As a result of their morphol
ogic heterogeneity, they can be confused easily with many tumors. Myoepithe
lial carcinomas have been underrecognized in the past, primarily by being l
umped under a broader category of "malignant mixed tumor." Awareness of the
ir unique cytoarchitectural patterns and immunohistochemical profile is cru
cial for accurate identification.