Induction of parturition with prostaglandin F-2 alpha as a possible model to study impaired reproductive performance in the dairy cow

Parturitions were induced in five cows, 2 weeks before term using prostagla ndin (PG) F-2 alpha. Two i.m, injections were performed with an interval of 24 h. All cows calved within 5 days (average 2.7 days) after the first inj ection of PGF(2 alpha). Out of five cows, four had retained fetal membranes (RFM), Each animal was sampled for bacteriological examination using uteri ne biopsies twice a week during 42 days postpartum (PP). Jugular vein blood samples were withdrawn for PGF(2 alpha)-metabolite and progesterone analys es five times per day during the first week PP and eight times per 24 h dur ing the 2nd and 3rd weeks PP. From the 4th week, the sampling interval was reduced back to five times per day. From the 5th week PP, the sampling was reduced to two times per day and sampling was terminated after day 46 PP. O nly morning samples were used for progesterone analyses, From day LO PP, ul trasonography (US) was performed every 3rd day until day 39 PP for detectio n of ovarian activity and follicular dynamics. The highest incidence of bac teriological species was found during the first 3 weeks PP. After the 5th w eek of collection, all animals were free from bacteria. The species of bact eria found were Arcanobacterium (Actinomyces) pyogenes, Escherichia coli, a lpha-hemolytic streptococcae and Pasteurella multocida. Immediately after p arturition, very high levels of the PG-metabolite were seen in all animals, with a sharp decrease to line of significance around days 9-12 PP, Small i ncreases above the line of significance were detected up to day 27 PP in co ws with RFM, and after that time the levels were considered to be at baseli ne. Low levels of progesterone were seen in four animals during the whole e xperimental rime. Ln one animal, an increase was seen on day 43 PP, which w as maintained until the end of the experimental period on day 46 PP. Based on US, follicular waves were detected in all animals during the experimenta l period. In three animals, three non-ovulatory follicular waves were detec ted and in two animals, four non-ovulatory follicular waves were detected d uring 39 days of ultrasound sessions. Based on progesterone levels, only on e animal was considered to have ovulated around day 40 PP. Results from the present study indicate that reproductive performance of cows after PG-indu ced parturitions differs from those of spontaneous cases of RFM. Difference s regarding the resumption of ovarian activity were also observed between p revious studies of dexamethasone-induced parturitions and the present study . (C) 2000 Elsevier Science B.V. All rights reserved.