Intraspecific variation in Cannabis sativa L. based on intergenic spacer region of chloroplast DNA

We analyzed the nucleotide sequences of the non-coding region of chloroplas t DNA: the intergenic spacer between trnL (UAA) 3'exon and trnF (GAA). Two kinds of sequence, "type-1" and "type-2," were detected in 33 populations o f Cannabis sativa. The length of the "type-1" fragment was 354 bp. In contr ast, the "type-2" fragment from 3 populations was 353 bp long, with only on e base deletion compared to "type-1" The fragment length from Humulus lupul us was 353 bp with a 1-bp deletion, and ten 1-bp substitutions compared to the sequences from C. sativa "type-1". Furthermore, we could clearly identi fy differences between C. saliva and H. lupulus using single-strand conform ation polymorphism of PCR products (PCR-SSCP) analysis.