AFM characterization of protein net formation on a fibrous medium

Lysozyme protein net. is set on a glass fiber support using the self-assemb ly technique. Enzymatic film formation is followed by surface imaging via a tomic force microscopy (AFM). Change in roughness as a function of depositi on time is used as an indirect indicator of film formation. The objective w as to form a protein film that would have no effect on the permeability of the medium, aiming at its application as a bioactive membrane or reactor su itable for bacteria and chemical interactions in aqueous media.