Plant molecular responses to osmotic stress are complex as evidenced b
y the isolation of numerous OR (osmotic stress-regulated) genes. Altho
ugh functions including osmolyte biosynthesis, membrane transport, sig
nal transduction, and cellular protection have been predicted for OR g
enes, few of them have been established. Current efforts toward isolat
ing and analyzing the expression of individual OR genes should be repl
aced by systematic approaches to analyze all OR genes simultaneously i
n selected plant species. Both transcriptional and posttranscriptional
regulation of OR genes have been described. Cis-elements that respond
to osmotic stress through abscisic acid (ABA)-dependent as well as AB
A-independent pathways have been identified. Functional genetic approa
ches using yeast and plant model systems are expected to complement cu
rrent molecular analysis of overexpression of OR genes in transgenic p
lants. These systems will help to establish functions of OR genes, to
dissect osmotic stress-signaling pathways, and to determine critical a
nd rate-limiting cellular processes for osmotic stress tolerance. In t
his regard, initial results obtained through mutational analysis in Ar
abidopsis thaliana are promising and have identified novel salt-tolera
nt as well as salt-hypersensitive mutants.