VETERINARY DRUG RESIDUES SURVEY IN MEAT - AN HPLC METHOD WITH A MATRIX SOLID-PHASE DISPERSION EXTRACTION

A multiresidue technique for the isolation and liquid chromatography d etermination of 14 veterinary drug residues in meat is described (10 a ntibiotics, 2 anthelmintics, 1 coccidiostat, and 1 other chemotherapeu tic drug). Matrix solid phase dispersion (MSPD) was chosen as the extr action technique for this screening method. Two fractions were collect ed by elution with methylene chloride and ethyl acetate. No additional purification was needed. A reversed-phase C18 column was used for the high performance liquid chromatography (HPLC) to separate the analyte s with gradients of ammonium acetate buffer-acetonitrile-methanol as m obile phase. Photodiode array and fluorimetric detectors were used for this analysis. Validation data are presented. The limits of detection for each analyte are shown and are under the regulatory tolerances. T he percentage recoveries and the linearity of the method were evaluate d comparing spiked samples and standard solutions. Meat naturally cont aminated with sulfamethazine was used for the evaluation of the repeat ability of the method, which was satisfactory.