Tmm. Malundo et al., METHOD FOR THE RAPID HEADSPACE ANALYSIS OF MANGO (MANGIFERA-INDICA L)HOMOGENATE VOLATILE CONSTITUENTS AND FACTORS AFFECTING QUANTITATIVE RESULTS, Journal of agricultural and food chemistry, 45(6), 1997, pp. 2187-2194
A rapid GC method was developed to analyze the headspace composition o
f cultivar Kent, Keitt, and Tommy Atkins mango homogenates. Factors af
fecting quantitative results were also studied. Of the 13 volatile com
pounds identified, 9 were terpene hydrocarbons: alpha-pinene, beta-pin
ene, 3-carene, myrcene, limonene, p-cymene, terpinolene, alpha-copaene
, and caryophyllene. Volatile concentrations were quantified using pea
k height calibration curves (peak height vs concentration). Linear rel
ationships were derived for all compounds except caryophyllene. Rate o
f peak height increase was slower at caryophyllene levels <2 ppm than
at those greater than or equal to 2 ppm. Volatile levels increased as
homogenate was diluted with up to 56% water. Addition of CaCl2 during
blending, to inhibit degradative enzymes, induced gelation, which resu
lted in decreased volatile concentrations in the headspace. Sonicating
gelled homogenate resulted :in partial liquefaction and an increase i
n headspace volatiles. These results indicate that significant interac
tions between mango pulp and volatile compounds occur and, depending o
n sample preparation method, can affect quantitative results.