M. Cechowska-pasko et J. Palka, Age-dependent changes in glycosaminoglycan content in the skin of fasted rats. A possible mechanism, EXP TOX PAT, 52(2), 2000, pp. 127-131
It is well recognized that during fasting or aging of animals there is a de
creased content of several extracellular matrix components in the skin, inc
luding glycosaminoglycans (GAGs) and decrease in biosynthesis of these macr
omolecules. The mechanism for the phenomena is not known. We considered ski
n and blood lactate as a potential candidate to control GAG metabolism in t
issues. Energetic metabolism, reflected by NAD/NADH and lactate/pyruvate ra
tios is changed during aging or fasting and lactate inhibits at least some
GAGs biosynthesis. Therefore we have compared the level of lactate and the
ratios of lactate to pyruvate in the blood and skin of fasted young and fas
ted adult rats and correlated them with the content of skin glycosaminoglyc
ans. It has been found that the skin of adult rats contains about 60% of GA
Gs found in the skin of young animals. Fasting of both groups of animals re
sulted in further decrease in skin GAG content. GAG content in the skin of
fasted young animals was decreased by 30% while in fasted adult rats no sig
nificant differences were observed, compared to fed animals. Lactate concen
tration was found to be increased over 2-fold in the skin of young fasted r
ats, compared to young controls. The lactate concentration in adult animals
was not changed during fasting, although in both cases the lactate levels
were almost 3-fold higher than in young control rats. In blood, lactate con
centration increased by 40% during fasting of young animals while it decrea
sed by about 40% during fasting of adult rats. Although no differences were
found in blood lactate level between young and adult rats, the ratio of la
ctate/pyruvate was decreased by over 2 fold in adult rats. The relative dif
ferences in mean GAG content in the skin of all experimental groups of anim
als were related to the similar differences in blood glucose and lactate/py
ruvate ratio. Therefore not only skin lactate but also blood lactate concen
trations may reflect the extent of skin GAG biosynthesis. We have noticed t
hat increase in the ratio of skin lactate/pyruvate concentration and decrea
se of the ratio in the blood is accompanied by decrease in the skin GAG con
tent. We suggest that the phenomenon may result from utilization of lactate
into glucose in the Cori cycle which regulate glucose availability for GAG
biosynthesis. Therefore it can be suggested that lactate may participate i
n inhibition of skin GAG biosynthesis and the extent of the inhibition is r
eflected by the ratio of lactate/pyruvate concentrations both in the skin a
nd blood.