Chloromethane stimulates growth of Methylomicrobium album BG8 on methanol

Citation
Ji. Han et Jd. Semrau, Chloromethane stimulates growth of Methylomicrobium album BG8 on methanol, FEMS MICROB, 187(1), 2000, pp. 77-81
Citations number
12
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
03781097 → ACNP
Volume
187
Issue
1
Year of publication
2000
Pages
77 - 81
Database
ISI
SICI code
0378-1097(20000601)187:1<77:CSGOMA>2.0.ZU;2-W
Abstract
Studies were performed to determine if the growth of Methylomicrobium album BG8 on methanol could be enhanced through the provision of chloromethane. M. album BG8 was found to be able to oxidize chloromethane via the particul ate methane monooxygenase with an apparent K-s of 11+/-3 mu M and V-max of 15+/-0.6 nmol (min mg protein)(-1). When up to 2.6 mM chloromethane was pro vided with 5 mM methanol, methanotrophic growth was significantly enhanced in comparison to the absence of chloromethane, indicating that methanotroph s can utilize chloromethane to support growth, although it could not serve as a sole growth substrate. [C-14]chloromethane was found to be oxidized to [C-14]CO2 as well as incorporated into biomass. These results indicate tha t reactions previously thought to be cometabolic may actually provide some benefit to methanotrophs and that these cells can use multiple compounds to enhance growth. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.