Jf. Colombel et al., Genotypic analysis of thiopurine S-methyltransferase in patients with Crohn's disease and severe myelosuppression during azathioprine therapy, GASTROENTY, 118(6), 2000, pp. 1025-1030
Background & Aims: Myelosuppression in patients with Crohn's disease (CD) t
reated with azathioprine has been attributed to low activity of thiopurine
S-methyltransferase (TPMT), Allelic variants of the TPMT gene responsible f
or changes in the enzyme activity have been characterized. We investigated
the distribution of mutant alleles associated with TPMT deficiency in patie
nts with CD and myelosuppression during azathioprine/6-mercaptopurine thera
py. Methods: Forty-one patients with CD were included. They developed leuko
penia or thrombocytopenia during azathioprine or 6-mercaptopurine treatment
. Polymerase chain reaction-based methods were used to search for mutations
associated with TPMT deficiency, Results: Four patients (10%) had 2 mutant
alleles associated with TPMT deficiency, 7 (17%) had 1 mutant allele, and
30 (73%) had no known TPMT mutation. The delay between administration of th
e drug and occurrence of bone marrow toxicity was less than 1.5 months in t
he 4 patients with 2 mutant alleles, and ranged from 1 to 18 months in pati
ents with 1 mutant allele and from 0.5 to 87 months in patients with normal
genotype. Conclusions: Twenty-seven percent of patients with CD and myelos
uppression during azathioprine therapy had mutant alleles of the TPMT gene
associated with enzyme deficiency. Myelosuppression is more often caused by
other factors. Continued monitoring of blood cell counts remains mandatory
in patients treated with azathioprine.