CHARACTERIZATION OF A TRIMERIC COMPLEX CONTAINING OCT-1, SNAP(C), ANDDNA

The human small nuclear (su) RNA promoters contain a proximal sequence element (PSE), which recruits the basal transcription factor SNAP(c), and a distal sequence element characterized by an octamer sequence, w hich recruits the POU domain transcription factor Oct-1, The Oct-1 POU domain and SNAP(c) bind cooperatively to probes containing a PSE and an octamer sequence, and this effect contributes to efficient transcri ption in vitro. In vivo, however, Oct-1 regions outside of the POU dom ain can activate snRNA gene transcription. Here, we have examined whet her the role of these regions is to contribute to cooperative binding with SNAP(c). We find that they indeed improve cooperative binding, bu t most of the effect is nevertheless mediated by just the POU domain, This suggests that Oct-1 activates transcription of snRNA genes In at least two steps, recruitment of SNAP(c) mediated primarily by the POU domain, and a later step mediated by regions outside of the POU domain , We also show that a PSE-binding complex observed in nuclear extracts consists of Oct-1 and SNAP(c). Although Oct-1 cannot bind effectively to the PSE probe on its own, in the complex it contacts DNA, Thus, in a nuclear extract, SNAP(c) can recruit Oct-1 to a probe to which Oct- 1 cannot bind on its own.