A panel of CAb antibodies recognize endogenous and ectopically expressed ING1 protein

Nine monoclonal antibodies (MAbs) against human and rodent ING1 protein hav e been generated using an IL6-secreting mouse myeloma line. These antibodie s are all effective in recognizing ING1 protein in ELISAs, Western blot ass ays, and by indirect immunofluorescence. Combining different CAb monoclonal antibodies in a Western blot assay also allows detection of the very low l evels of endogenous ING1 found in fibroblast cells in culture and the ident ification of at least two isoforms of ING1 in normal human diploid fibrobla sts and established brain cancer cell lines.