K-Ras mutations and benign pancreatic disease

Citation
M. Lohr et al., K-Ras mutations and benign pancreatic disease, INT J PANCR, 27(2), 2000, pp. 93-103
Citations number
121
Categorie Soggetti
da verificare
Journal title
INTERNATIONAL JOURNAL OF PANCREATOLOGY
ISSN journal
01694197 → ACNP
Volume
27
Issue
2
Year of publication
2000
Pages
93 - 103
Database
ISI
SICI code
0169-4197(200004)27:2<93:KMABPD>2.0.ZU;2-W
Abstract
This review addresses the history of the ras oncogene, the techniques used to detect molecular alterations in the ras oncogene, and the application of polymerase chain reaction (PCB)-based methods to determine point mutations in clinical samples of patients with pancreatic diseases, namely pancreati c carcinoma and chronic pancreatitis, The frequency of res mutations in pan creatic carcinoma is high, ranging from 70 to almost 100%, The frequence sf res mutations in chronic pancreatitis, either in pancreatic tissue or panc reatic secretions, vary between 0 and 100%. This wide range in part may be swing to differences in sampling, DNA extraction, or PCR method, The meanin g of a k-ras mutation is under debate, Taking into account the positivity o f ductal hyperplasias in normal pancreas and ras mutations in normal appear ing duet: cells, this molecular finding may not mean anything, in contrast, res mutations are associated with smoking, one acknowledged risk factor fo r pancreatic carcinoma, The need for large prospective cohort studies is em phasized.