Relapsing and remitting experimental autoimmune encephalomyelitis in B cell deficient mice

Experimental autoimmune encephalomyelitis (EAE) is an animal model for the human autoimmune central nervous system (CNS) disease multiple sclerosis (M S). To examine the role of B cells in EAE with a relapsing and remitting di sease course (R-EAE) we generated (B10.PLxSJL/J)F1 mice deficient in B cell s by disrupting their heavy chain transmembrane region (B10.PLxSJL/J)F1 mu MT-/-. By immunizing (B10.PLxSJL/J)F1 and (B10.PLxSJL/J)F1 mu MT-/- mice wi th the encephalitogenic N-terminal peptide Acl-11 of myelin basic protein ( MBP), we observed that B-cell deficient mice exhibited a relapsing and remi tting disease course. Since a similar day of onset and day of first relapse were observed these data suggest that B cells do not play a vital role in the activation of T cells leading to the initiation of EAE, nor in the reac tivation of T cells resulting in R-EAE. (C) 2000 Academic Press.