Yeast nucleoporins involved in passive nuclear envelope permeability

The vertebrate nuclear pore complex (NPC) harbors an similar to 10-nm diame ter diffusion channel that is large enough to admit 50-kD polypeptides. We have analyzed the permeability properties of the Saccharomyces cerevisiae n uclear envelope (NE) using import (NLS) and export (NES) signal-containing green fluorescent protein (GFP) reporters. Compared with wild-type, passive export rates of a classical karyopherin/importin (Kap) Kap60p/Kap95p-targe ted NLS-GFP reporter (cNLS-GFP) were significantly faster in nup188-Delta a nd nup170-Delta cells. Similar results were obtained using two other NLS-GF P reporters, containing either the kap104p-targeted Nab2p NLS (rgNLS) or th e Kap121p-targeted Pho4p NLS (pNLS). Elevated levels of Hsp70 stimulated cN LS-GFP import, but had no effect on the import of rgNLS-GFP. Thus, the role of Hsp70 in NLS-directed import may be NLS- or targeting pathway-specific. Equilibrium sieving limits for the diffusion channel were assessed in vivo using NES-GFP reporters of 36-126 kD and were found to be greater than wil d-type in nup188-Delta and nup170-Delta cells. We propose that Nup170p and Nup188p are involved in establishing the functional resting diameter of the NPC's central transport channel.