Study of the binding of (99m)technetium-radiopharmaceuticals on blood cells and plasma proteins: Evaluation using precipitation with trichloroacetic acid.

Nuclear medicine uses radioactive tracers called radiopharmaceuticals to st udy the bloodflow, metabolism and morphology of an organ. Sodium pertechnet ate ((TcO4Na)-Tc-99m) and many Tc-99m products are the most frequently radi opharmaceuticals used in nuclear medicine. Secure determination of the bind ing of 99mTc, radiopharmaceuticals to plasma (P) and blood cell (BC) consti tuents can help to understand the biodistribution of radiopharmaceuticals. The reported evaluations about the binding of radiopharmaceuticals on blood elements have shown that the results can not be easily compared. We decide d to determine the gold standard concentration of trichloroacetic acid (TCA ) to study the binding of radiopharmaceuticals on brood proteins: Tc-99m-st annous colloid (Tc-99m-Sn-Colloid), sodium pertechnetate ((TcO4Na)-Tc-99m), methylenediphosphonic acid (Tc-99m-MDP) and diisopropyl iminodiacetic Copy right (C) 2000 John Wiley & Sons, Ltd.