A fast and simple method for the determination of clavulanic acid in humanplasma using derivatisation reaction kinetics

Authors
Ellis, H Hundt, HKL Swart, KJ Hundt, AF Joubert, AL van Essen, GH du Plessis, JB
Citation
H. Ellis et al., A fast and simple method for the determination of clavulanic acid in humanplasma using derivatisation reaction kinetics, J PHARM B, 22(6), 2000, pp. 933-937
Citations number
12
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN journal
07317085 → ACNP
Volume
22
Issue
6
Year of publication
2000
Pages
933 - 937
Database
ISI
SICI code
0731-7085(200007)22:6<933:AFASMF>2.0.ZU;2-A
Abstract
A stopped-flow mixing technique is used to determine clavulanic acid in hum an plasma after plasma deproteinisation with acetonitrile and removal of th e organic solvent by extraction with dichloromethane. The reaction kinetic profiles for the reaction between clavulanic acid and imidazole are determi ned by measuring the absorbance at 312 nm of the imidazole derivative. The reaction rates are proportional to the concentration of the clavulanic acid and by plotting reaction rates against clavulanic acid concentrations line ar calibration curves could be constructed over the range 0.30-10.0 mu g/ml . (C) 2000 Elsevier Science B.V. All rights reserved.