Reactivity of monoclonal antibodies against a tryptophan-riboflavin adducttoward irradiated and non-irradiated bovine-eye-lens protein fractions: anindicator of in vivo visible-light-mediated phototransformations

We describe here the reactivity toward the soluble protein of bovine eye le ns of anti-tryptophan-riboflavin ( anti-Trp-RF) adduct monoclonal antibodie s, which recognize the hapten typtophan-riboflavin generated by irradiation of a solution of bovine serum albumin in the presence of riboflavin. It is demonstrated that five different anti-Trp-RF adduct monoclonal antibodies, all belonging to the IgG(1) isotype, react with the total soluble proteins of bovine eye lens. The components of the soluble protein are separated by Sephadex G-200 chromatography and the isolated fractions analyzed by sodiu m dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). All the se parated protein fractions also react by a direct ELISA with the monoclonal antibodies; this reaction is more intense when the isolated fractions have been previously irradiated with visible light in the presence of riboflavin under an atmosphere of oxygen or nitrogen. Irradiation of the total solubl e protein with visible light in the presence of riboflavin produces the app earance of new bands, corresponding to compounds of higher molecular weight . Riboflavin-sensitized irradiation of the protein fractions with visible l ight under an oxygen or nitrogen atmosphere is accompanied by a concomitant decrease of the tryptophan fluorescence. It is postulated that the action of visible light in the presence of either the endogenous riboflavin or its derivatives could be partly responsible for the protein aggregation observ ed during aging. (C) 2000 Elsevier Science S.A, All rights reserved.