Unwinding of a herpes simplex virus type 1 origin of replication (Ori(S)) by a complex of the viral origin binding protein and the single-stranded DNA binding protein

Authors
He, XD Lehman, IR
Citation
Xd. He et Ir. Lehman, Unwinding of a herpes simplex virus type 1 origin of replication (Ori(S)) by a complex of the viral origin binding protein and the single-stranded DNA binding protein, J VIROLOGY, 74(12), 2000, pp. 5726-5728
Citations number
11
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
74
Issue
12
Year of publication
2000
Pages
5726 - 5728
Database
ISI
SICI code
0022-538X(200006)74:12<5726:UOAHSV>2.0.ZU;2-H
Abstract
A herpes simplex virus type 1 (HSV-1) Ori(S) analogue in which the A+T sequ ence linking: the box I and II elements was replaced by two single-stranded oligo(dT)s is unwound by the UL9 protein ICP8 complex. Unwinding of wild-t ype Ori(S) by the UL9 protein-ICP8 complex was also observed under conditio ns which destabilize the A+T sequence. These experiments support a model fo r the unwinding of Ori(S) in which destabilization of the A+T sequence can generate a single-stranded DNA binding site for ICP8, which then associates with the UL9 protein bound to boxes I and II to promote the bidirectional unwinding of Ori(S).