R. Hofbauer et al., Prostaglandin E-1 is able to increase migration of leukocytes through endothelial cell monolayers, MICROVASC R, 59(3), 2000, pp. 354-360
Leukocyte interactions with endothelial cells play an important role during
inflammatory processes. Leukocytes pass a monolayer of endothelial cells (
ECM) to migrate into the extravascular space. The aim of the current study
was to investigate whether prostaglandin E-1 (PGE(1)) influences the proces
s of leukocyte migration. In a prospective controlled study, the influence
of prostaglandin E-1 (50-5000 ng/mL) on leukocyte migration through endothe
lial cell monolayers (n = 7) was investigated. Human umbilical endothelial
cells (HUVEC) and/or leukocytes were preincubated with clinically relevant,
higher, and lower concentrations of prostaglandin E-1 and the amount of le
ukocyte migration after 3 h was measured. HUVEC were cultured on microporou
s membrane filters until achievement of a monolayer for investigation of le
ukocyte migration. Polymorphonuclear leukocytes (PMNL) were isolated from h
ealthy volunteers and PMNL migration was studied under the influence of PGE
(1). In clinically relevant concentrations, PGE(1) was able to increase sig
nificantly leukocyte migration through endothelial cell monolayers (205 +/-
7.8%, P < 0.05 compared to control; when treating PMNL alone, migration ra
te was 120 +/- 9.2% compared to control, ns; only endothelial cell monolaye
rs treated up to 145 +/- 10.2%, P < 0.05 compared to control) showing a dos
e-dependent effect. In this assay, both cell types (PMNL and ECM) could be
treated simultaneously, simulating the clinical situation after an iv admin
istration. In conclusion, PGE(1) is able to increase leukocyte migration th
rough endothelial cell monolayers when both cell types are pretreated. The
treatment of either leukocytes or endothelial cell monolayers in the cell c
oculture showed no significant increase. These findings support the theory
that prostaglandins may play a major role during inflammation. Future clini
cal studies are warranted to confirm this hypothesis. (C) Academic Press.