Multiplex PCR detection of Campylobacter jejuni and Arcobacter butzleri infood products

Arcobacter is a recently described species, previously considered part of t he Campylobacter family. A sensitive assay such as that provided by PCR cou ld help to distinguish the closely related Arcobacter from Campylobacter. A PCR method to specifically detect both Campylobacter jejuni and Arcobacter butzleri in the same reaction tube has been developed. C. jejuni and A. bu tzleri were inoculated into a range of dairy products, raw and ready-to-eat foods. The presence of these two organisms was detected in these test food s by the multiplex PCR assay. A product of 159 bp was apparent when C. jeju ni was present, while a 1223 bp product was seen when A. butzleri was prese nt. When both organisms were present, both bands could be detected on the a garose gel. All organisms were confirmed by standard microbiological method s. There was complete agreement between the PCR and standard methods. This PCR assay will allow detection of both organisms within the same PCR tube a nd can be performed within an 8 h day. The presence of these two human path ogens, which are difficult to distinguish by standard biochemical methods, can now be identified using this PCR assay. (C) 2000 Academic Press.