Induction of the ATP-sensitive potassium (uk(ATP)-1) channel by endotoxemia

The effect of sepsis on the ubiquitously expressed ATP-sensitive potassium (uK(ATP)-1) channel expression was measured in Sprague-Dawley rat diaphragm s. Rats were treated with either 0.5 mi saline or 20 mg/Kg E. coli lipopoly saccharides and sacrificed at 3, 6, 12, 24, or 48 h later. Reverse transcri ptase-polymerase chain reaction (RT-PCR) analysis showed that channel mRNA expression was increased at 3 h and continued to rise up to 48 h. Western b lotting analysis showed a similar to 9-fold increase in channel protein exp ression 24 h after sepsis. Our results demonstrate that sepsis upregulates the uK(ATP)-1 channel. (C) 2000 John Wiley & Sons, Inc.