Identification of cyst forming nematodes of the genus Heterodera (Nematoda: Heteroderidae) based on the ribosomal DNA-RFLP

Authors
Subbotin, SA Waeyenberge, L Moens, M
Citation
Sa. Subbotin et al., Identification of cyst forming nematodes of the genus Heterodera (Nematoda: Heteroderidae) based on the ribosomal DNA-RFLP, NEMATOLOGY, 2, 2000, pp. 153-164
Citations number
33
Categorie Soggetti
Animal Sciences
Journal title
NEMATOLOGY
ISSN journal
13885545 → ACNP
Volume
2
Year of publication
2000
Part
2
Pages
153 - 164
Database
ISI
SICI code
1388-5545(2000)2:<153:IOCFNO>2.0.ZU;2-B
Abstract
Amplified ITS region products of rDNA from 25 valid species and one unident ified species from the genus Heterodera and from Meloidodera alni were dige sted by 26 restriction enzymes. A combination of seven enzymes clearly sepa rated the agriculturally most important species from each other and from th eir sibling species. Species specific digestion profiles of ITS regions and a table with approximate sizes of digested fragments for several identific ation enzymes are given. Heterogeneity of ITS regions was revealed for some Cyst forming nematode species.