Heterogeneity of dipeptidyl peptidase IV from C6 rat glioma cells

Dipeptidyl peptidase IV is known to be involved, due to both hydrolytic and non-hydrolytic mechanisms, in various cell functions of normal and cancer cells as well. In this report dipeptidyl peptidase IV substrate and pH pref erences, some inhibition parameters, freezing/thawing sensitivity and stabi lity against hydrolysis by trypsin were studied in C6 rat glioma cells. Our results confirmed substantial heterogeneity of dipeptidyl peptidase IV pop ulation. Such observation is important to avoid methodological artifacts an d to decrease risk of misinterpretations in biological studies.