Paraquat-induced lipid peroxidation and injury in Ehrlich ascites tumor cells

Citation
P. Karakashev et al., Paraquat-induced lipid peroxidation and injury in Ehrlich ascites tumor cells, NEOPLASMA, 47(2), 2000, pp. 122-124
Citations number
15
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
NEOPLASMA
ISSN journal
00282685 → ACNP
Volume
47
Issue
2
Year of publication
2000
Pages
122 - 124
Database
ISI
SICI code
0028-2685(2000)47:2<122:PLPAII>2.0.ZU;2-A
Abstract
The participation of lipid peroxidation products in the mechanisms of paraq uat toxicity in Ehrlich ascites tumor (EAT) cells was observed. Paraquat in a concentration 0.5-1.0 mmol increased the level of lipid peroxidation acc ording to the Ohakawa TBARS (thiobarbituric acid-reactive substances) metho d. These changes in TBARS production in EAT cells correlated with paraquat toxicity on the cells registered by using the method for cell injury, which is based on changes in lactate dehydrogenase activity. The metal chelator DTA removed the effect of paraquat on TBARS production and on cell injury. The present data suggested that the increased level of lipid peroxidation a nd cell injury is a result of the paraquat action in EAT cells depending on iron.