Altered expression of tissue-type plasminogen activator and type 1 inhibitor in astrocytes of mouse cortex following scratch injury in culture

Citation
Hb. Tang et al., Altered expression of tissue-type plasminogen activator and type 1 inhibitor in astrocytes of mouse cortex following scratch injury in culture, NEUROSCI L, 285(2), 2000, pp. 143-146
Citations number
19
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE LETTERS
ISSN journal
03043940 → ACNP
Volume
285
Issue
2
Year of publication
2000
Pages
143 - 146
Database
ISI
SICI code
0304-3940(20000512)285:2<143:AEOTPA>2.0.ZU;2-2
Abstract
The expression of plasminogen and plasminogen activators (PG/PAs) in reacti ve astrocytes was examined following scratch injury. In response to injury, casein-degrading activity could be observed around astrocytes. The protein expression of tissue-type plasminogen activator (tPA) was up-regulated, wh ile the free form of urokinase-type plasminogen activator (uPA) was not det ected. Consistent with these findings, results obtained with zymograph assa y also revealed that tPA activity, but not uPA activity, was up-regulated. Moreover, the addition of 6-amino-caproitic acid (EACA) to casein-covered a strocytes significantly prevented the recovery of the injured astrocytes in a dose-dependent manner. Taken together, our data demonstrate that the exp ression of PG/PAs in cultured astrocytes is regulated following injury, sug gesting that caseinolytic activity is an essential component during the pro cess of astrocyte recovery. (C) 2000 Elsevier Science Ireland Ltd. All righ ts reserved.