Essential moiety for antimutagenic and cytotoxic activity of hederagenin monodesmosides and bisdesmosides isolated from the stem bark of Kalopanax pictus
Kt. Lee et al., Essential moiety for antimutagenic and cytotoxic activity of hederagenin monodesmosides and bisdesmosides isolated from the stem bark of Kalopanax pictus, PLANTA MED, 66(4), 2000, pp. 329-332
For the elucidation of the antimutagenic and cytotoxic principles from the
stem bark of Kalopanax pictus, seven isolated components of this crude drug
were tested in the Ames test and the MTT test. Hederagenin and its monodes
mosides, kalopanaxsaponin A and I in addition to its bisdesmosides, kalopan
axsaponin B and H, showed potent antimutagenic activities against aflatoxin
B-1 (AFB(1)). However, they had no inhibitory effects on mutagenicity indu
ced by the direct mutagen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Thi
s suggested that hederagenin glycosides might effectively prevent the metab
olic activation of AFB(1) or scavenge the electrophilic intermediate capabl
e of inducing mutation. Hederagenin was found to be an essential moiety for
the exhibition of antimutagenicity. Moreover, hederagenin and its 3-O-glyc
osides were found to be cytotoxic on various tumor cell lines, P-388, L-121
0, U-937, HL-60, SNU-5 and HepG2, while 3,28-di-O-glycosides of hederagenin
were not cytotoxic. Hence, hederagenin and its 3-O-glycosides could be sui
table for cancer treatment chemopreventive drugs.