Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric detection of bacterial biomarker proteins isolated from contaminated water, lettuce and cotton cloth

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectra of bacterial proteins were obtained from water, lettuce and cloth samples contaminated with Shigella flexneri, Escherichia coli, and Aeromona s hydrophila. Spectra were obtained using proteins directly isolated from w ater (or water used for rinsing samples) without culturing the bacteria. Fo r S, flexneri and E, coli, two marker ions for specific proteins associated with a virulence-related property (acid resistance) were easily detected. For A, hydrophila, ions from two specifically selected marker proteins, as well as ions from the larger group of proteins isolated from pure cultures, all matched spectra from a contaminated water sample, providing strong evi dence that A. hydrophila was the bacterial contaminant, Rinse water from co ntaminated lettuce and cloth samples showed the same marker ions as the con taminated water samples, Copyright (C) 2000 John Wiley & Sons, Ltd.