Correction for quenching in fluorimetric determinations using steady statefluorescence

Quenching corrections in fluorimetric estimations are arrived at using time resolved fluorometry, where the reduction in the lifetimes of the fluoroph ore in the presence of quenchers is a measure of the correction. A novel pr ocedure for the correction of quenching in fluorimetric determinations usin g steady state fluorescence spectroscopy is described hen. The method is ba sed on the variation in the slope of the fluorescence versus concentration plots, as the quencher concentration is changed. As a test case, the proced ure for the determination of uranium has been demonstrated in the presence of a number of metal ion quenchers. (C) 2000 Elsevier Science B.V. All righ ts reserved.