Quenching corrections in fluorimetric estimations are arrived at using time
resolved fluorometry, where the reduction in the lifetimes of the fluoroph
ore in the presence of quenchers is a measure of the correction. A novel pr
ocedure for the correction of quenching in fluorimetric determinations usin
g steady state fluorescence spectroscopy is described hen. The method is ba
sed on the variation in the slope of the fluorescence versus concentration
plots, as the quencher concentration is changed. As a test case, the proced
ure for the determination of uranium has been demonstrated in the presence
of a number of metal ion quenchers. (C) 2000 Elsevier Science B.V. All righ
ts reserved.