Optimization in peptide synthetic conditions of 1,4-butanediol dimethacrylate cross-linked polystyrene resin and its efficiency in solid phase peptide synthesis

A 1,4-butanediol dimethacrylate cross-linked polystyrene (PS-BDODMA) suppor t was prepared by aqueous suspension polymerization. The C-terminal amino a cid incorporation, N-alpha-Fmoc and Boc-deprotection, acylation reactions a nd the removal of the target peptide from the support were optimized. The e fficiency of the support was demonstrated by synthesizing leucyl-alanyl-gly cyl-valine, acyl carrier protein fragment (65-74) and a 25-residue peptide fragment designed from the NS1 region of hepatitis C viral polyprotein unde r optimal reaction conditions. The efficiency of the polymer support was co mpared with commercially available Merrifield and Sheppard resins by synthe sizing the same peptides under the identical conditions. The purity of thes e peptides was checked by HPLC and the structures of the peptides were esta blished by amino acid analysis and MALDI TOF MS. (C) 2000 Elsevier Science Ltd. All rights reserved.