Novel HLA-B*15 alleles identified in potential marrow donors

Citation
Tm. Williams et al., Novel HLA-B*15 alleles identified in potential marrow donors, TISSUE ANTI, 55(5), 2000, pp. 455-459
Citations number
10
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
TISSUE ANTIGENS
ISSN journal
00012815 → ACNP
Volume
55
Issue
5
Year of publication
2000
Pages
455 - 459
Database
ISI
SICI code
0001-2815(200005)55:5<455:NHAIIP>2.0.ZU;2-2
Abstract
Several methods for low-resolution class I typing of potential bone marrow donors are available. The National Marrow Donor Program(R) (NMDP) has initi ated pilot projects for large-scale DNA-based class I typing to initially c haracterize donors. Sequence-specific oligonucleotide probe hybridization a nd sequence-specific primer polymerase chain reaction (PCR) screening of 3, 500 NMDP potential donors suggested the presence of variants of known HLA-B *15 variants in 3 donors. PCR products encompassing HLA-B locus exons 1 thr ough 3 were prepared and subcloned. Sequencing revealed 3 alleles differing from known HLA-B*15 alleles by nucleotide substitutions resulting in predi cted novel HLA-B antigens. The new alleles occur in distinct ethnic groups. These findings further illustrate the substantial genetic variation presen t at the HLA-B locus within human populations.