Relation among serum and tissue concentrations of lutein and zeaxanthin and macular pigment density

Background: Lutein and zeaxanthin are the only carotenoids in the macular r egion of the retina (referred to as macular pigment [MP]). Foods that are r ich in lutein and zeaxanthin can increase MP density. Response to dietary l utein and zeaxanthin in other tissues has not been studied. Objective: The objective of this study was to examine tissue responses to d ietary lutein and zeaxanthin and relations among tissues in lutein and zeax anthin concentrations. Design: Seven subjects consumed spinach and corn, which contain lutein and zeaxanthin, with their daily diets for 15 wk. At 0, 4, 8, and 15 wk and 2 m o after the study, serum, buccal mucosa cells, and adipose tissue were anal yzed for carotenoids, and MP density was measured. Results: Serum and buccal cell concentrations of lutein increased significa ntly from baseline during dietary modification. Serum zeaxanthin concentrat ions were greater than at baseline only at 4 wk, whereas buccal cell and ad ipose tissue concentrations of zeaxanthin did not change. Adipose tissue lu tein concentrations peaked at 8 wk. Changes in adipose tissue lutein concen tration were inversely related to the changes in MP density, suggesting an interaction between adipose tissue and retina in lutein metabolism. To inve stigate the possibility of tissue interactions, we examined cross-sectional relations among serum, tissue, and dietary lutein concentrations, anthropo metric measures, and MP density in healthy adults. Significant negative cor relations were found between adipose tissue lutein concentrations and MP fo r women, but a significant positive relation was found for men. Conclusion: Sex differences in lutein metabolism may be an important factor in tissue interactions and in determining MP density.