Ratiometric oxygen sensing: detection of dual-emission ratio through a single emission filter

Authors
Kostov, Y Harms, P Pilato, RS Rao, G
Citation
Y. Kostov et al., Ratiometric oxygen sensing: detection of dual-emission ratio through a single emission filter, ANALYST, 125(6), 2000, pp. 1175-1178
Citations number
55
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYST
ISSN journal
00032654 → ACNP
Volume
125
Issue
6
Year of publication
2000
Pages
1175 - 1178
Database
ISI
SICI code
0003-2654(2000)125:6<1175:ROSDOD>2.0.ZU;2-I
Abstract
A new method and device for the ratiometric measurement of oxygen concentra tion are presented. They are based on the use of a dual-emission oxygen-sen sitive dye. The method allows the exclusion of the influence of emission ov erlap. The detection of the dual-emission ratio is performed using a single long-pass emission filter. The device described is simpler than the widely used lifetime instruments and could easily be a stand-alone low-cost instr ument.