H-point curve isolation method for coupled liquid chromatography and UV-visible spectrophotometry

The H-point curve isolation method (HPCIM) for the detection of unknown int erferences in chromatography is proposed. The method allows one to estimate the UV-vis spectra of interfering species in a sample as well as to test t he purity of the chromatographic peaks. Besides the detection of the unknow n interferences in a sample, this method allows one to calculate the concen tration of an analyte in the presence of unknown compounds. To illustrate t he reliability of the proposed method, samples of diuretics and amphetamine s have been analyzed by normal- and reversed-phase high-performance chromat ography.