H-point curve isolation method for coupled liquid chromatography and UV-visible spectrophotometry

Citation
F. Blasco-gomez et al., H-point curve isolation method for coupled liquid chromatography and UV-visible spectrophotometry, ANALYT CHEM, 72(11), 2000, pp. 2559-2565
Citations number
21
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
00032700 → ACNP
Volume
72
Issue
11
Year of publication
2000
Pages
2559 - 2565
Database
ISI
SICI code
0003-2700(20000601)72:11<2559:HCIMFC>2.0.ZU;2-R
Abstract
The H-point curve isolation method (HPCIM) for the detection of unknown int erferences in chromatography is proposed. The method allows one to estimate the UV-vis spectra of interfering species in a sample as well as to test t he purity of the chromatographic peaks. Besides the detection of the unknow n interferences in a sample, this method allows one to calculate the concen tration of an analyte in the presence of unknown compounds. To illustrate t he reliability of the proposed method, samples of diuretics and amphetamine s have been analyzed by normal- and reversed-phase high-performance chromat ography.