F. Blasco-gomez et al., H-point curve isolation method for coupled liquid chromatography and UV-visible spectrophotometry, ANALYT CHEM, 72(11), 2000, pp. 2559-2565
The H-point curve isolation method (HPCIM) for the detection of unknown int
erferences in chromatography is proposed. The method allows one to estimate
the UV-vis spectra of interfering species in a sample as well as to test t
he purity of the chromatographic peaks. Besides the detection of the unknow
n interferences in a sample, this method allows one to calculate the concen
tration of an analyte in the presence of unknown compounds. To illustrate t
he reliability of the proposed method, samples of diuretics and amphetamine
s have been analyzed by normal- and reversed-phase high-performance chromat
ography.