Sequence and transfection of BoLA-DRB3 cDNAs

Authors
Russell, GC Fraser, DC Craigmile, S Oliver, RA Dutia, BM Glass, EJ
Citation
Gc. Russell et al., Sequence and transfection of BoLA-DRB3 cDNAs, ANIM GENET, 31(3), 2000, pp. 219-222
Citations number
20
Categorie Soggetti
Animal Sciences","Molecular Biology & Genetics
Journal title
ANIMAL GENETICS
ISSN journal
02689146 → ACNP
Volume
31
Issue
3
Year of publication
2000
Pages
219 - 222
Database
ISI
SICI code
0268-9146(200006)31:3<219:SATOBC>2.0.ZU;2-Q
Abstract
Bovine MHC (BoLA-) DRB3 alleles encoded by the DH8A, DH22A and DH24A class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other full-length DRB3 sequences suggested that DRB3 allel es may have evolved through multiple lineages. All three BoLA-DRB3 alleles were shown to express on the surface of transfected cells, and the transfec tants were used to define or confirm the class II specificity of a panel of monoclonal antibodies.