G. Ameye et al., The value of interphase fluorescence in situ hybridization for the detection of translocation t(12;21) in childhood acute lymphoblastic leukemia, ANN HEMATOL, 79(5), 2000, pp. 259-268
Translocation t(12;21)(p13;q22) is the most frequent cytogenetic abnormalit
y in childhood acute lymphoblastic leukemia (ALL) and is generally associat
ed with favorable prognosis. In this report, we assessed the value of dual-
color interphase fluorescence in situ hybridization (FISH) for the detectio
n of t(12;21). Fifty-three patients were screened for ETV6/CBFA2 fusion by
means of FISH, using two cosmid probes mapped on ETV6 and on CBFA2, respect
ively. The cut-off value (mean + three standard deviations) for positivity
established on control patients was 9.3%. A comparison between FISH and mol
ecular methods [reverse-transcriptase polymerase chain reaction/Southern bl
ot (RT-PCR/SB)] was possible in 52 patients: 34 of 52 (65.4%) showed negati
ve results with both approaches, and 13 of 52 (25%) were positive; 5 of 52
(9.6%) showed discrepancies: four patients who were positive using RT-PCR/S
B were negative using FISH. Conversely, one patient negative when using RT-
PCR/SB was positive with FISH. Further investigations on this patient, cyto
genetically characterized by add(lap), showed an atypical breakpoint on ETV
6, located 5' to the common breakpoint. Compared with RT-PCR and SE, dual-c
olor interphase FISH with the cosmid probe set proved to be highly specific
but showed limited sensitivity.