H. Kashiwase et al., Investigation into the mode of action of R-91650, an arylpiperazinyl fluoroquinolone, on feline immunodeficiency virus replication inhibitory activity, ARCH VIROL, 145(5), 2000, pp. 859-869
The mode of action of R-91650, an arylpiperazinyl fluoroquinolone, on felin
e immunodeficiency virus (FIV) replication inhibitory activity was investig
ated. R-91650 inhibited replication of FIV at non-cytotoxic concentration l
evels in both acutely infected peripheral blood mononuclear cells and chron
ically infected P-CrFK cells. The compound reduced the intracellular p24 co
ncentration levels in P-CrFK cells in a dose-dependent manner. Northern blo
t analysis revealed that R-91650 selectively prevented the accumulation of
FIV mRNA in P-CrFK cells. However, the compound did not inhibit FIV-long te
rminal repeat (LTR) promoter activity in the reporter gene expression analy
sis. These data suggest that R-91650 is a novel inhibitor of FIV replicatio
n that inhibits a certain step or steps following transcription initiation
of the FIV-LTR promoter.