Intraoperative sentinel lymph node examination by imprint cytology and frozen sectioning during breast surgery

Background: The purpose of the present study was to evaluate the usefulness of intraoperative imprint cytology and frozen sectioning of sentinel lymph nodes in patients with clinically node-negative breast cancer. Methods: Sentinel node biopsy was performed in 101 patients with stage I or II breast cancer with clinically negative nodes using a dye-guided method. Intraoperative evaluation of sentinel node involvement by imprint cytology and frozen sectioning was compared with the final histopathological result s of permanent sections. Tumour-negative nodes in paraffin sections stained by haematoxylin and eosin were further studied using an anticytokeratin an tibody. Results: The results of imprint cytology and frozen-section analysis were c ompared with those of haematoxylin and eosin-stained sections. The sensitiv ity, specificity and overall accuracy of imprint cytology were 96.0, 90.8 a nd 92.1 per cent respectively, and those of frozen-section examination were 52.0, 100 and 88.1 per cent. Ten sentinel nodes were tumour positive on im print cytology and tumour negative on stained paraffin sections. Micrometas tasis was found in eight of these nodes on immunohistochemistry. Taking the se immunohistological results into consideration, the final sensitivity, sp ecificity and overall accuracy of imprint cytology were 90.9, 98.5 and 96.0 per cent respectively. Conclusion: Intraoperative imprint cytology is a useful method for evaluati ng the status of sentinel nodes and is more accurate than frozen-section an alysis. In addition, imprint cytology can detect micrometastasis more accur ately than conventional haematoxylin and eosin-stained sectioning.