Sp. Zhao et Dy. Xu, Oxidized lipoprotein(a) increases the expression of platelet-derived growth factor-B in human umbilical vein endothelial cells, CLIN CHIM A, 296(1-2), 2000, pp. 121-133
Lipoprotein(a) [Lp(a)] has been demonstrated to be an independent risk fact
or for atherosclerosis and restenosis after angioplasty. However, the preci
se mechanism by which it contributes to the development of both remains unc
lear. Platelet-derived growth factor (PDGF) is one of the key factors that
induce the proliferation and migration of vascular smooth muscle cells. The
present study investigated the effects of native and oxidized LDL [n-LDL a
nd ox-LDL] and Lp(a) [n-Lp(a) and ox-Lp(a)] on the expression of PDGF-B in
cultured human umbilical vein endothelial cells (HUVECs). Results showed th
at PDGF-B expression was not influenced by n-LDL, but was moderately increa
sed by ox-LDL and n-Lp(a). Ox-Lp(a) was the most potent stimulus for PDGF-B
expression, increasing it in HUVECs by 156%+/-18% at 5 nmol/l and 219%+/-4
2% at 20 nmol/l. Northern Blot analysis demonstrated that the amount of PDG
F-B mRNA was markedly increased after treatment with ox-Lp(a) but not n-LDL
, ox-LDL and n-Lp(a). These results demonstrate that ox-Lp(a) can elicit PD
GF-B expression in HUVECs, which may thereby influence the pathogenesis of
atherosclerosis and restenosis after angioplasty. (C) 2000 Elsevier Science
BN. All rights reserved.