Cloning of the Atlantic salmon (Salmo salar) estrogen receptor-alpha gene

Authors
Rogers, SA Llewellyn, L Wigham, T Sweeney, GE
Citation
Sa. Rogers et al., Cloning of the Atlantic salmon (Salmo salar) estrogen receptor-alpha gene, COMP BIOC B, 125(3), 2000, pp. 379-385
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN journal
03050491 → ACNP
Volume
125
Issue
3
Year of publication
2000
Pages
379 - 385
Database
ISI
SICI code
0305-0491(200003)125:3<379:COTAS(>2.0.ZU;2-W
Abstract
A cDNA clone encoding most of an Atlantic salmon (Salmo salar) estrogen rec eptor (ER) was obtained from a liver cDNA library and the remainder of the coding sequence from the gene was isolated from a genomic library. Sequence comparisons showed that the cloned gene represents ER-alpha. Expression of the ER-alpha gene in male and female salmon parr was analysed by RT-PCR. H ighest expression was found in brain and liver, with lower levels of ER-alp ha mRNA present in all other tissues tested. There was little difference in expression of ER-alpha between male and female. (C) 2000 Elsevier Science Inc. All rights reserved.