Td. Grant et al., Col2-GFP reporter marks chondrocyte lineage and chondrogenesis during mouse skeletal development, DEV DYNAM, 218(2), 2000, pp. 394-400
Mice were generated in which a Col2-GFP transgene serves as a reporter for
the chondrocyte lineage and for chondrogenesis in live embryos and newborn
pups. Cells actively engaged in chondrogenesis were identified by confocal
optical sectioning within their native environments in embryos and in thick
tissue slices. Chondrocytes exhibiting GFP fluorescence were purified from
rib cages by high-speed cell sorting of crude cell suspensions. Intensity
of fluorescence correlated with biosynthesis of procollagen II in these cel
ls. The use of these mice and their cells provides a novel approach for stu
dying chondrocyte differentiation and chondrogenesis during skeletal develo
pment. Dev Dyn 2000;218:394-400. (C) 2000 Wiley-Liss, Inc.