Application of sodium dodecyl sulfate micellar electrokinetic chromatography (SDS MEKC) for the rapid measurement of aqueous phase 2,4,6-trinitrotoluene metabolites in anaerobic sludge: A comparison with LC/MS

The present study describes the application of sodium dodecyl sulfate micel lar electrokinetic chromatography (SDS MEKC) for the fast analysis of TNT b iotransformation products in the aqueous phase of anaerobic sludge cultures . SDS MEKC was performed using a Hewlett-Packard HP 3D CE capillary electro phoresis system with photodiode array detection. In a single sample injecti on the SDS MEKC method detected 2,4,6-trinitrotoluene (TNT), 2-hydroxylamin o-4,6-dinitrotoluene (2-HADNT), 4-hydroxylamino-2,6-dinitrotoluene (4-HADNT ), 2-amino-4,6-dinitrotoluene (2-ADNT), 4-amino-2,6-dinitrotoluene (4-ADNT) , 2,4 -diaminonitrotoluene (2,4-DANT), 2,6-diaminonitrotoluene (2,6-DANT), and 2,4,6-triaminotoluene (TAT). Analyte peaks were verified using visible/ ultraviolet photodiode array spectra from commercial standards and periodic analyses using electrospray ionization liquid chromatography mass spectrom etry (ES-LC-MS). A time course study constructed from the SDS MEKC data pro vided supporting evidence for the stepwise reduction of TNT to TAT. The SDS MEKC method was judged to be of practical value for the identification of polar and moderately polar TNT biotransformants in place of HPLC and LC-MS methods, with significant reduction in material expense and analysis time.